| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Pasteurella multocida | AGATGCCGTTGTGGTGGA | TGCAGAACGCCAACCTGT | 58.84 | 59.81 | 250 | 140 |
100.00%
|
100.00%
|
| Primer 2 | Pasteurella multocida | AATGACCGACGCAGAGTGG | TCAGCGGATGCCAGTCAAA | 60.08 | 59.63 | 290 | 137 |
100.00%
|
100.00%
|
| Primer 3 | Pasteurella multocida | TGGCGATCACGATTGGTGC | TGGCGTTTAAGCTGACGTG | 61.11 | 58.77 | 196 | 135 |
100.00%
|
100.00%
|
| Primer 4 | Pasteurella multocida | AATGACCGACGCAGAGTGG | ATCAGCGGATGCCAGTCA | 60.08 | 58.69 | 291 | 132 |
100.00%
|
100.00%
|
| Primer 5 | Pasteurella multocida | AATGACCGACGCAGAGTGG | AATCAGCGGATGCCAGTCA | 60.08 | 59.40 | 292 | 132 |
100.00%
|
100.00%
|
| Primer 6 | Pasteurella multocida | GGGGTGAAGACTTGCGTCA | GCCCAAATAATCGCAGCCG | 59.93 | 60.01 | 224 | 132 |
100.00%
|
100.00%
|
| Primer 7 | Pasteurella multocida | GGGGTGAAGACTTGCGTCA | CAGCCGTCGTTAAGCCTGA | 59.93 | 60.08 | 211 | 132 |
100.00%
|
100.00%
|
| Primer 8 | Pasteurella multocida | AATGACCGACGCAGAGTGG | TCAGCGGATGCCAGTCAA | 60.08 | 58.93 | 290 | 130 |
100.00%
|
100.00%
|
| Primer 9 | Pasteurella multocida | AATGACCGACGCAGAGTGG | ATCAGCGGATGCCAGTCAA | 60.08 | 59.40 | 291 | 130 |
100.00%
|
100.00%
|
| Primer 10 | Pasteurella multocida | AAGATGCCGTTGTGGTGGA | TGCAGAACGCCAACCTGT | 59.55 | 59.81 | 251 | 129 |
100.00%
|
100.00%
|